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Image Search Results
Journal: Molecular Psychiatry
Article Title: The bacterial peptidoglycan-sensing molecule Pglyrp2 modulates brain development and behavior
doi: 10.1038/mp.2016.182
Figure Lengend Snippet: Expression and cellular localization of PGRPs in the developing brain. Changes in striatal Pglyrp2 ( a ) and Pglyrp3 ( b ) protein levels during postnatal development. Protein levels were normalized to the Hsp90 protein levels and expressed relative to the adult group (that is, P60; n =4 per group). All data ( a and b ) are presented as means (±s.e.m.). * P <0.05, ** P <0.01 and *** P <0.001 when compared with their respective adult groups (that is, P60). Distribution of Pglyrp2 in the cerebellum ( c ), frontal cortex ( d – f ) and hippocampus ( g – i ) at P3. Double immunofluorescence staining shows staining of Pglyrp2 (green) combined with either NeuN (a neuronal marker; red), Iba1 (a microglial marker; red), GFAP (an astrocyte marker; red) or calbindin (a marker for purkinje cells; red). The merged images show the colocalization of Pglyrp2 (green) and NeuN (red) in the frontal cortex ( d ) and hippocampus ( g ) and with calbindin (red) in the cerebellum ( c ). Note that Pglyrp2 is moderately colocalization with astrocytes ( f and i ), and with microglia to a lesser extend ( e and h ). Arrows indicate Pglyrp2-positive Purkinje cells (c), Pglyrp2-positive microglia ( e and h ) and Pglyrp2-positive astrocytes ( f and i ). The scale bars represent 50 μm. Hsp, heat shock protein; P, postnatal day; PGRP, peptidoglycan-recognition protein.
Article Snippet: For double labelling, the following antisera were used: mouse monoclonal antibody against NeuN to label mature neurons (cat no. MAB377, 1:250; Merck Millipore, Billerica, MA, USA),
Techniques: Expressing, Double Immunofluorescence Staining, Staining, Marker